Miguel Paredes-Barrada

The paper systematically engineers and analyzes intrinsic terminators in bacteria, finding that an adenosine upstream of the terminator hairpin improves protein production and reduces read-through.

The paper engineers Parageobacillus thermoglucosidasius to use methanol via the RuMP cycle for growth, achieving 17% biomass from methanol without heterologous enzyme expression.

This study attempts to engineer the reductive glycine pathway in Parageobacillus thermoglucosidasius for formate and methanol assimilation but finds limited success due to metabolic limitations and incomplete pathway implementation.