Brent E. Stead

A buffer-tuning strategy to profile domain-specific activity of chimeric I-TevI/CRISPR gene editors in vitro

This study shows how buffer conditions affect cleavage preferences of chimeric gene editors, revealing non-targeted cleavage in low salt environments.

Harnessing RNA-based DNA repair pathways for targeted gene editing

This paper introduces a method for targeted gene editing using RNA-templated DNA repair pathways in human cells, achieving high efficiency and fidelity.