Author

Paulius Toliusis

Vilnius UniversityORCID 0009-0006-6096-7346Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Approach:experimental assay· 2 Approach:biophysical modeling· 1 Research Focus:cell type annotation· 1 Research Focus:chromatin & epigenetic regulation· 1 Research Focus:protein folding & dynamics· 1 Assay:proteomics· 1 Assay:structural biology· 1

Frequent Co-Authors

Algirdas GrybauskasiD· 2 shared Mindaugas ZarembaiD· 2 shared Arūnas ŠilanskasiD· 1 shared Aurimas KopūstasiD· 1 shared Česlovas VenclovasiD· 1 shared E. ManakovaiD· 1 shared Edvinas JurgelaitisiD· 1 shared Evelina ZagorskaitėiD· 1 shared

Activity by Year

2025-2026 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 38 mutual co-author links

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2 shared papersBiochemistry

Algirdas GrybauskasORCID

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2 papers

Recent·Most cited

BiochemistrySynthetic BiologyPeer-reviewedJan 1, 2026

Off-target detection of CRISPR-Cas9 nuclease in vitro with CROFT-Seq

Paulius Toliusis, Algirdas Grybauskas, Tomas Šinkūnas…·Mindaugas Zaremba

This paper introduces CROFT-Seq, a method for detecting Cas9 off-target sites in vitro, which is sensitive, rapid, and cost-effective compared to existing methods.

3 citations

experimental assay

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BiochemistrybioRxivPreprintApr 8, 2025

Activation of SPARDA defense system by filament assembly reveals beta-relay signaling mechanism widespread in prokaryotic Argonautes

Edvinas Jurgelaitis, Evelina Zagorskaitė, Aurimas Kopūstas…·Mindaugas Zaremba

The paper reveals a beta-relay signaling mechanism in prokaryotic Argonautes that activates the SPARDA defense system to degrade invading DNA and host DNA, preventing plasmid spread.

2 citations

structural biologyproteomics

𝕏 PostBluesky