Mindaugas Zaremba

This paper introduces CROFT-Seq, a method for detecting Cas9 off-target sites in vitro, which is sensitive, rapid, and cost-effective compared to existing methods.

The paper reveals a beta-relay signaling mechanism in prokaryotic Argonautes that activates the SPARDA defense system to degrade invading DNA and host DNA, preventing plasmid spread.

The paper identifies His-ADPR as a novel TIR-derived immune signaling molecule in bacteria, showing its role in phage defense and structural basis for recognition.

The paper shows short prokaryotic Argonautes, when associated with Sir2, deplete NAD+ to defend against invading DNA.