This study assesses adult structural plasticity in Drosophila neurons by examining remodeling processes and introducing a tool to measure complexity without observer bias.

Open Biology · Sep 9, 2026Peer-reviewedNeuroscience

Adapting Upright Light Sheet Fluorescence Microscopy for Imaging at Air-Liquid Interface

Chad Michael Hobson, Kenji Izumi, Jesse Aaron, Navaneetha Krishnan Bharathan, M. Fernanda Ceriani, W. Giang, Juan Ignacio Ispizua, Andrew P. Kowalczyk, Rachel Lee, E. Angelo Morales, Owen F. Puls, Ellen K. Quarles, Micaela Rodríguez-Caron, Sara N. Stahley, Francisco Tassara, , Teng‐Leong Chew

This paper presents a device for adapting upright light sheet fluorescence microscopy to image samples at air-liquid interface, demonstrating its use on three biological specimens.

npj Imaging · Jul 22, 2026Peer-reviewedBioengineering

Daily ultrastructural remodeling of clock neurons

Juan Ignacio Ispizua, Micaela Rodríguez-Caron, Francisco Joaquín Tassara, Keun-Young Kim, Christian Mauricio Carpio-Romero, Christi Perkins, Milagros Barzi, Miguel F. Vasquez, Celia Napier Hansen, Julián Gargiulo, Ezio Rosato, Horacio O. de la Iglesia, Mark H. Ellisman, M. Fernanda Ceriani

The paper examines daily structural changes in Drosophila clock neurons using SBEM, revealing rhythmic variations in morphology, synaptic markers, and mitochondrial structure.

Current Biology · Nov 19, 2025Peer-reviewedNeuroscience

Single Objective Light Sheet Microscopy allows high-resolution in vivo brain imaging of Drosophila.

Francisco Joaquín Tassara, Mariano Barella, Lourdes Simó, Mailen Folgueira, Micaela Rodríguez-Caron, Juan Ignacio Ispizua, Mark H. Ellisman, Horacio O. de la Iglesia, M. Fernanda Ceriani, Julián Gargiulo

This paper introduces a single objective light-sheet microscope for high-resolution in vivo brain imaging in Drosophila, achieving improved resolution and reduced photobleaching and phototoxicity.

bioRxiv · Nov 6, 2024 · PreprintNeuroscience