Cell BiologyExperimental Eye ResearchPeer-reviewed
Reassessing Retinal Pigment Epithelial Ketogenesis: Enzymatic Assays for Ketone Body Levels Provide Inaccurate Results
Gillian A Gulette, Daniel T. Hass, Kriti Pandey…·Jason M. L. Miller
This study shows that enzymatic assays overestimate β-hydroxybutyrate (β-HB) in RPE cultures, while mass spectrometry reveals equal apical and basal secretion and β-HB sources from fatty acids and amino acids.
5 citations