Author

Melissa P. Tumen-Velasquez

Oak Ridge National LaboratoryORCID 0009-0009-1634-4055Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Genetics· 1 Synthetic Biology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 2 Organism:E. coli· 1

Frequent Co-Authors

Adam M. GussiD· 2 shared Andrew P. HreniD· 1 shared Brian F. PflegeriD· 1 shared Carrie A. EckertiD· 1 shared Irenee C. PayneiD· 1 shared Jerome M. FoxiD· 1 shared Joshua P. AbrahamiD· 1 shared Lauren A. Riley· 1 shared

Activity by Year

2023-2024 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 31 mutual co-author links

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2 shared papersSynthetic Biology

Adam M. GussORCID

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2 papers

Recent·Most cited

Synthetic BiologybioRxivPreprintSep 17, 2024

High-efficiency transformation and gene expression in Picosynechococcus sp. PCC 7002

Andrew P. Hren, Joshua P. Abraham, Melissa P. Tumen-Velasquez…·Carrie A. Eckert

This study improves gene expression and transformation efficiency in Picosynechococcus sp. PCC 7002 by optimizing protocols and identifying a better integration site.

2 citations

experimental assay

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GeneticsJournal of BacteriologyPeer-reviewedJan 19, 2023

Simple and rapid site-specific integration of multiple heterologous DNAs into the Escherichia coli chromosome

Lauren A. Riley, Irenee C. Payne, Melissa P. Tumen-Velasquez, Adam M. Guss

The paper develops a system for rapid, site-specific integration of multiple DNA constructs into the E. coli chromosome using recombinases, demonstrating its utility by inserting DNA methyltransferases to enable transformation of Clostridium clariflavum.

23 citations

E. coliexperimental assay

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