Brian F. Pfleger

High-efficiency transformation and gene expression in Picosynechococcus sp. PCC 7002

This study improves gene expression and transformation efficiency in Picosynechococcus sp. PCC 7002 by optimizing protocols and identifying a better integration site.

Model-guided design of the diversity of a synthetic human gut community

This paper develops a model-guided framework to design high-diversity synthetic human gut communities by optimizing culture conditions and leveraging ecological models.

EnZymClass: Substrate specificity prediction tool of plant acyl-ACP thioesterases based on Ensemble Learning

EnZymClass predicts plant acyl-ACP thioesterase substrate specificity using ensemble learning, achieving high accuracy and outperforming existing methods.

Machine learning-guided acyl-ACP reductase engineering for improved in vivo fatty alcohol production

This study uses machine learning to engineer acyl-ACP reductases for improved in vivo fatty alcohol production, achieving over twofold higher titers than natural enzymes.

Construction and Operation of an Affordable Laboratory Photobioreactor System for Simultaneous Cultivation of up to 12 Independent 1 L Cyanobacterial Cultures

The paper describes the design and construction of an affordable photobioreactor system capable of cultivating up to 12 independent 1 L cyanobacterial cultures at a cost lower than a single commercial unit.