Alicia-Sophie Schebesta

The paper introduces pSPEC, a reproducible proteomic sample preparation method for biofluids that increases protein identification by ~20% compared to in-solution plasma.

This study evaluates bead-based and conventional plasma proteomics workflows, finding bead methods enhance low-abundance protein detection but are prone to contamination bias, while perchloric acid-based methods resist contamination better.

This study evaluates whether chromatographic performance affects proteome coverage under high-speed data acquisition, finding that proteome depth is now more influenced by mass spectrometry than chromatography.