Lyl-1 regulates primitive macrophages and microglia development

Abstract

During ontogeny, macrophages (M{Phi}) populations emerge in the Yolk Sac (YS) via two distinct progenitor waves, prior to hematopoietic stem cell development. M{Phi}-progenitors from the primitive/"early EMP" and transient-definitive/"late EMP" waves both contribute to various resident-M{Phi} populations in the developing embryonic organs. Identifying factors that modulates early stages of M{Phi}-progenitor development may lead to a better understanding of defective function of specific resident-M{Phi} subsets. Here we show that primitive macrophage (M{Phi}Prim) progenitors in the YS express Lyl-1, a bHLH transcription factor related to SCL/Tal-1. Transcriptomic analysis of YS M{Phi}-progenitors indicated that M{Phi}Prim progenitors present at embryonic day (E) 9 are clearly distinct from those present at later stages. Disruption of Lyl-1 basic helix-loop-helix domain led initially to an early increased emergence of M{Phi}Prim progenitors, and later to their defective differentiation. These defects were associated with a disrupted expression of gene sets related to embryonic patterning and neurodevelopment. Lyl-1-deficiency also induced a reduced production of mature M{Phi}/microglia in the early brain, as well as a transient reduction of the microglia pool at midgestation and in the newborn. We thus identify Lyl-1 as a critical regulator of M{Phi}Prim and microglia development, which disruption may impair resident-M{Phi} function during organogenesis. Key points1- Yolk sac primitive macrophage progenitors and microglia/Border Associated macrophages express Lyl-1. 2- Lyl-1-deficiency impairs primitive macrophage and microglia development and leads to the up-regulation of gene sets related to embryo patterning and neuro-development.