A Beauty Dye Staining (ABDS) - inexpensive marker-ink based open-source alternative for commercial membrane vital dyes

In this paper, we aim to present a new intravital dye, called ABDS, which can be prepared using a marker pen and is useful for eukaryotic cell research. ABDS binds to the cytoplasmic membrane of living cells and can be used for their visualization in biophysical experiments, including Z-stack tomography, cell proliferation monitoring, and adhesion studies. Important properties of ABDS are its availability, bright stable fluorescence, manufacturing simplicity, and safety for living cells in vitro. The paper includes a method for preparing ABDS, a data set with its characteristics, as well as examples of ABDS use in cells investigation.