FluoVolt Staining Induces Photodamage During Live-Cell Voltage Imaging
Evren M. Akyuz, Manuela Mitroi, Fleur E Groualle, Foteini Patera, Ruman Rahman, Stuart J. Smith, Ian Spendlove, Judith M. Ramage, Hester Franks, Andrew Mark Jackson, Adam Mark Blanchard, Anna A. Malecka, Frankie James Rawson
This study shows FluoVolt staining and excitation cause photodamage and cell perturbation in live-cell voltage imaging, with recommendations to reduce dye concentration and loading time.
bioRxiv · Jun 11, 2026 · PreprintBiophysics