bioRxivBioengineering
Instantaneous isotropic volumetric imaging of fast biological processes
N. Wagner, N. Norlin, J. Gierten, G. de Medeiros, B. Balazs, J. Wittbrodt, L. Hufnagel, R. Prevedel
Abstract
Capturing highly dynamic biological processes at sub-cellular resolution is a recurring challenge in biology. Here we show that combining selective volume illumination with simultaneous acquisition of orthogonal light-fields yields 3D images with high, isotropic spatial resolution and free of reconstruction artefacts, thereby overcoming current limitations of light-field microscopy implementations. We demonstrate Medaka heart and blood flow imaging with single-cell resolution and free of motion artefacts at volume rates up to 200Hz.
- organism: zebrafish
- assay: imaging
- research focus: cell type annotation
- approach: microscopy & imaging analysis
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