Classification of electrophysiological and morphological types in mouse visual cortex

Nathan W. Gouwens; Staci A. Sorensen; Jim Berg; Changkyu Lee; Tim Jarsky; Jonathan T. Ting; Susan M. Sunkin; David H. Feng; Costas A. Anastassiou; Eliza R. Barkan; Kris Bickley; Nicole Blesie; Thomas Braun; Krissy Brouner; Agata Budzillo; Shiella Caldejon; Tamara Casper; Daniel Castelli; Peter Chong; Kirsten Crichton; Christine Cuhaciyan; Tanya L. Daigle; Rachel A. Dalley; Nick Dee; Tsega Desta; Song‐Lin Ding; Samuel Dingman; Alyse Doperalski; Nadezhda I. Dotson; Tom Egdorf

doi:10.1038/s41593-019-0417-0

NeuroscienceNature NeuroscienceJun 17, 2019

Peer-reviewed journal article

Classification of electrophysiological and morphological types in mouse visual cortex

Nathan W. Gouwens iD, Staci A. Sorensen iD, Jim Berg iD, Changkyu Lee iD, Tim Jarsky iD, Jonathan T. Ting iD, Susan M. Sunkin iD, David H. Feng iD, Costas A. Anastassiou iD, Eliza R. Barkan iD, Kris Bickley iD, Nicole Blesie, Thomas Braun iD, Krissy Brouner iD, Agata Budzillo iD, Shiella Caldejon iD, Tamara Casper iD, Daniel Castelli, Peter Chong iD, Kirsten Crichton iD, Christine Cuhaciyan, Tanya L. Daigle iD, Rachel A. Dalley iD, Nick Dee iD, Tsega Desta iD, , Christof Koch A iD

Abstract

Understanding the diversity of cell types in the brain has been an enduring challenge and requires detailed characterization of individual neurons in multiple dimensions. To profile morpho-electric properties of mammalian neurons systematically, we established a single cell characterization pipeline using standardized patch clamp recordings in brain slices and biocytin-based neuronal reconstructions. We built a publicly-accessible online database, the Allen Cell Types Database, to display these data sets. Intrinsic physiological and morphological properties were measured from over 1,800 neurons from the adult laboratory mouse visual cortex. Quantitative features were used to classify neurons into distinct types using unsupervised methods. We establish a taxonomy of morphologically- and electrophysiologically-defined cell types for this region of cortex with 17 e-types and 35 m-types, as well as an initial correspondence with previously-defined transcriptomic cell types using the same transgenic mouse lines.

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