bioRxivBioengineering
An adaptive optics module for deep tissue multiphoton imaging in vivo
C. Rodriguez, A. Chen, J. A. Rivera, M. A. Mohr, Y. Liang, W. Sun, D. E. Milkie, T. G. Bifano, X. Chen, N. Ji
Abstract
Understanding complex biological systems requires visualizing structures and processes deep within living organisms. We developed a compact adaptive optics module and incorporated it into two- and three-photon fluorescence microscopes, to measure and correct tissue-induced aberrations. We resolved synaptic structures in deep cortical and subcortical areas of the mouse brain, and demonstrated high-resolution imaging of neuronal structures and somatosensory-evoked calcium responses in the mouse spinal cord at unprecedented depths in vivo.
- organism: mouse
- assay: imaging
- research focus: cell type annotation
- research focus: spatial domain detection
- approach: experimental assay
- approach: microscopy & imaging analysis
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