Genome ResearchGenomicsPeer-reviewed journal article
WHOLE GENOME BISULPHITE SEQUENCING USING THE ILLUMINA HISEQ X SYSTEM
Masako SuzukiiD, Will LiaoiD, Frank Wos, Andrew D. JohnstoniD, Justin DeGraziaiD, Jennifer K. Ishii, Toby Bloom, Michael C. ZodyiD, Søren GermeriD, John M. GreallyiD
Abstract
The Illumina HiSeq X platform has helped to reduce the cost of whole genome sequencing substantially, but its application for bisulphite sequencing is not straightforward. We describe the optimization of a library preparation and sequencing approach that maximizes the yield and quality of sequencing, and the elimination of a previously unrecognized artefact affecting several percent of bisulphite sequencing reads.
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