A cellular stress response induced by the CRISPR/dCas9 activation system is not heritable through cell divisions
Andrew D. Johnston, Alali Abdulrazak, Hanae Sato…·Claudia Avello Simões‐Pires
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Albert Einstein College of MedicineORCID 0000-0002-5769-3106Unclaimed ORCID profile · follow to get notified of new preprints
4 papers
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Andrew D. Johnston, Alali Abdulrazak, Hanae Sato…·Claudia Avello Simões‐Pires
Andrew D. Johnston, Claudia Avello Simões‐Pires, Masako Suzuki, John M. Greally
This paper describes a protocol for efficient CRISPR-mediated genomic editing in Epstein-Barr virus-transformed lymphoblastoid B cells using ssODN, achieving 20% successful editing and identifying structural mutations missed by targeted sequencing.
Masako Suzuki, Will Liao, Frank Wos…·John M. Greally
The paper optimizes library preparation and sequencing for whole genome bisulphite sequencing on the Illumina HiSeq X system, eliminating an artefact affecting several percent of reads.
Netha Ulahannan, Masako Suzuki, Claudia Avello Simões‐Pires…·John M. Greally
This study investigates how Toxoplasma gondii infection alters host transcriptional regulation by redistributing AP-1 transcription factor binding, leading to changes in chromatin accessibility and gene expression.