JNK signaling-mediated exocytosis coordinates epithelial cell-turnover in Drosophila ribosomal protein mutants

Abstract

Robust tissue growth is orchestrated by the precise coordination of cell death and cell proliferation. Our previous study found that in the developing wing pouches of Drosophila Minute/+ animals, both cell death and compensatory cell proliferation are dramatically increased, which contributes to robust growth of mutant tissue. The induction of this cell-turnover depends on activation of JNK signaling, although the mechanism by which JNK activation induces cell-turnover remained unclear. Here, we show that JNK-mediated elevation of exocytosis in dying cells is crucial for triggering cell-turnover in M/+ wing morphogenesis. Mechanistically, elevated JNK signaling in dying cells upregulates exocytosis-related genes and Wingless (Wg), leading to enhanced Wg secretion. Furthermore, this exocytosis-mediated Wg secretion generally occurs downstream of JNK signaling, regardless of the genetic background. Overall, our findings provide mechanistic insights into robust tissue growth through the orchestration of cell-turnover, which is primarily governed by JNK-mediated exocytosis during Drosophila Minute/+ wing morphogenesis.