Long-term effects of early postnatal stress on Sertoli cells functions

Abstract

Sertoli cells are somatic cells in testes essential for spermatogenesis, as they support the development, maturation, and differentiation of germ cells. Sertoli cells are metabolically highly active and physiologically regulated by external signals, particularly factors in the blood stream. In disease conditions, circulating pathological signals may affect Sertoli cells and consequentially, alter germ cells and fertility. While the effects of stress on reproductive cells have been well studied, how Sertoli cells respond to stress remains poorly characterized. Therefore, we used a mouse model of early postnatal stress to assess the effects of stress on Sertoli cells. We developed an improved enrichment strategy based on intracellular stainings and obtained enriched preparations of adult Sertoli cells from exposed males. We show that adult Sertoli cells have impaired electron transport chain (ETC) pathways and that several components of ETC complexes I, III, and IV are persistently affected. We identify the circulation as a potential mediator of the effects of stress, since treatment of primary Sertoli cells with serum from stressed males induces similar ETC alterations. These results newly highlight Sertoli cells as cellular targets of early life stress, and suggest that they may contribute to the negative effects of stress on fertility. HighlightsO_LIWe present an improved method to obtain enriched preparations of Sertoli cells from adult mouse testis for molecular analyses C_LIO_LISertoli cells from adult males exposed to stress during early postnatal life have altered electron transport chain (ETC) expression, suggesting persistent effects of early life stress on Sertoli cells physiology C_LIO_LISerum from adult males exposed to early postnatal stress reproduces ETC gene dysregulation in cultured Sertoli cells. C_LI Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=147 SRC="FIGDIR/small/501498v1_ufig1.gif" ALT="Figure 1"> View larger version (54K): org.highwire.dtl.DTLVardef@ba92e9org.highwire.dtl.DTLVardef@1032032org.highwire.dtl.DTLVardef@36bf72org.highwire.dtl.DTLVardef@52e3d6_HPS_FORMAT_FIGEXP M_FIG C_FIG