bioRxivBioengineeringPreprint
An in-vitro BBB-on-a-chip open model of human blood-brain barrier enabling advanced optical imaging
M. Salman, G. Marsh, I. Kusters, M. Delince, G. Di Caprio, S. Upadhyayula, G. De Nola, R. Hunt, K. G. Ohashi, F. Shimizu, Y. Sano, T. Kanda, B. Obermeier, T. Kirchhausen
Abstract
ABSTRACTWe describe here the design and implementation of an in-vitro BBB-on-a-chip open model system capable of reconstituting the microenvironment of the blood brain barrier. This system allows controlled unidirectional flow of nutrients and biologicals on the lumen of the artificial microvessel. This BBB-on-a-chip is suitable for high resolution electron microscopy and it is amenable for quantitative 3D live fluorescence imaging using spinning confocal disk or lattice light sheet microscopy (LLSM) to follow, for example the transcytosis across the BBB-like barrier of fluorescently-tagged biological, viruses or nanoparticles.Competing Interest StatementG.M. and B.O. are employees and shareholders of Biogen. T.K. is a visiting scientist at Biogen.View Full Text
- organism: human
- assay: imaging
- research focus: cell type annotation
- research focus: cell signaling & trafficking
- approach: microscopy & imaging analysis
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- Hypoxia-enhanced Blood-Brain Barrier Chip recapitulates human barrier function, drug penetration, and antibody shuttling properties
Tae-Eun Park, Nur Mustafaoglu, … Donald E. Ingber·bioRxiv·Nov 29, 2018
- Lab-on-a-chip device integrating a human stem cell-based blood-brain barrier model with neural organoids for translational research
Judit P. Vigh, Anna E. Kocsis, … Fruzsina R. Walter·bioRxiv·Sep 17, 2026
- Building the blood-brain barrier: a scalable self-assembling 3D model of the brain microvasculature under unidirectional flow
Jade Admiraal, Promise Emeh, … Nienke R. Wevers·Fluids and Barriers of the CNSPeer-reviewed·Jan 23, 2026
- A physiological microfluidic blood-brain-barrier model for in vitro study of nanoparticle trafficking and accumulation
Bryan B. Nguyen, Neona M. Lowe, … Randy P. Carney·Advanced Healthcare MaterialsPeer-reviewed·Mar 21, 2026
- Establishment of murine in vitro blood-brain barrier models using immortalized cell lines: co-cultures of brain endothelial cells, astrocytes, and neurons
Fakhriedzwan Idris, Siti Hanna Muharram, … Suwarni Diah·bioRxiv·Oct 5, 2018
- A Human Brain-Chip for modelling brain pathologies and screening of BBB crossing therapeutic strategies
Shek Man Chim, Kristen Howell, … Elias Pavlopoulos·PharmaceuticsPeer-reviewed·Oct 10, 2024