T. Kirchhausen

Constitutive Endolysosomal Perforation in Neurons allows Induction of alpha-Synuclein Aggregation by Internalized Pre-Formed Fibrils

The paper shows that neurons have constitutive endolysosomal perforations enabling internalized alpha-synuclein fibrils to induce aggregation, and inhibiting PIKfyve kinase reduces this effect.

Deep learning assisted single particle tracking for automated correlation between diffusion and function

This paper introduces DeepSPT, a deep learning framework that automates the analysis of sub-cellular diffusion to infer functional information, achieving high accuracy in identifying endosomal structures during viral infections.

Temporal Dynamics and Stoichiometry in Notch Signaling - from Notch Synaptic Complex Formation to NICD Nuclear Entry

This study tracks Notch and Delta interactions in real-time, showing 1:1 stoichiometry at synapses and NICD nuclear entry after 45 minutes, inhibited by signaling blockers.

Complete Protection from SARS-CoV-2 Lung Infection in Mice Through Combined Intranasal Delivery of PIKfyve Kinase and TMPRSS2 Protease Inhibitors

This study shows that combining PIKfyve and TMPRSS2 inhibitors intranasally protects mice from SARS-CoV-2 lung infection and reduces cell death.

Probing weak lipid-lipid binding domain interactions with a mechanically transduced immunosorbent assay (METRIS)

This paper introduces METRIS, a new assay to measure weak protein-lipid interactions, and demonstrates its application to study PIP-binding domains with varying affinities.

Nanomolar inhibition of SARS-CoV-2 infection by an unmodified peptide targeting the pre-hairpin intermediate of the spike protein

Deep neural network automated segmentation of cellular structures in volume electron microscopy

This paper introduces ASEM, a deep learning pipeline for automated segmentation of cellular structures in electron microscopy, demonstrating its effectiveness in identifying various organelles and membrane structures.

Humanized antibody potently neutralizes all SARS-CoV-2 variants by a novel mechanism

The paper describes a humanized antibody, SP1-77, that neutralizes all SARS-CoV-2 variants by blocking membrane fusion instead of ACE2 receptor binding.

SARS-CoV-2 requires acidic pH to infect cells

This study shows SARS-CoV-2 requires acidic pH for membrane fusion and genome release, even after proteolytic cleavage by furin or TMPRSS2.

A serotonergic axon-cilium synapse drives nuclear signaling to maintain chromatin accessibility

The paper identifies axo-ciliary synapses that mediate serotonin signaling to regulate chromatin accessibility in hippocampal neurons.

Synergistic block of SARS-CoV-2 infection by combined drug inhibition of the host entry factors PIKfyve kinase and TMPRSS2 protease

This study finds that combining apilimod and camostat mesylate or nafamostat mesylate significantly enhances inhibition of SARS-CoV-2 infection in vitro.

Cholesterol 25-hydroxylase suppresses SARS-CoV-2 replication by blocking membrane fusion

The paper identifies CH25H and its product 25HC as inhibitors of SARS-CoV-2 replication by blocking membrane fusion.

An in-vitro BBB-on-a-chip open model of human blood-brain barrier enabling advanced optical imaging

The paper describes an in-vitro BBB-on-a-chip model that enables high-resolution imaging of blood-brain barrier dynamics.

Inhibition of PIKfyve kinase prevents infection by Zaire ebolavirus and SARS-CoV-2

The paper shows that PIKfyve kinase inhibitors block infection by Zaire ebolavirus and SARS-CoV-2 by preventing viral content release.

Correlative three-dimensional super-resolution and block face electron microscopy of whole vitreously frozen cells

This study develops a platform for 3D correlative cryogenic super-resolution and block-face EM of whole vitreously frozen cells, revealing new protein-ultrastructure relationships.

Dynamics of Auxilin1 and GAK in clathrin-1 mediated traffic

The paper shows Aux1 and GAK are recruited in transient bursts to clathrin-coated vesicles, with their timing linked to phosphoinositide changes, suggesting a lipid-switch mechanism for uncoating.