Nature CatalysisSynthetic BiologyPeer-reviewed journal article
Engineered Enzymes that Retain and Regenerate their Cofactors Enable Continuous-Flow Biocatalysis
Carol Janet HartleyiD, Charlotte C. WilliamsiD, Judith A. ScobleiD, Quentin I. ChurchesiD, Andrea J. NorthiD, Nigel G. French, Thomas NebliD, Greg Coia, Andrew C. WardeniD, Greg Simpson, Andrew R. Frazer, Chantel Nixon Jensen, Nicholas John TurneriD, Colin Stephen ScottiD
Abstract
Introduction Introduction Results Conclusions Author Contributions Competing Interests Statement General DNA manipulation G3PDEc NoxCa GlpKTk AceKMs GlpKTk-AceKMs G3PDEc-NOXCa GlpKTk-AceKMsE2Aa G3PDEc-NOXCa-Est2Aa FruASc-Est2Aa Engineered Fusion Protein... Expression of individual enzymes... Expression of GlpKTk-AceKMs-E2Aa Expression and purification of... Expression and purification of... Protein Expression and... Synthesis of N6-2AE-ADP ...
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