Patterned photocatalytic proximity labelling for spatial interactomics

Mapping protein-protein interactions and proximity within their native spatial context is essential for elucidating molecular mechanisms underlying biological processes; however, methods for spatial interactomics with subanatomical resolution are limited. Here, we introduce digital micromirror device (DMD)-PhoxID, a method that combines photocatalytic proximity labelling with patterned irradiation using a DMD to map the proximal molecular environment of proteins of interest within defined subregions of fixed brain tissue.