bioRxivMicrobiologyPreprint
Isothermal Detection of Influenza D using RT-LAMP
Carlos Abelardo dos Santos, Jialu Li, Pauline M. van DiemeniD, Andrew McMahoniD, Benjamin C. MollettiD, Andrew M. RamsayiD, Meshach MainaiD, Joe JamesiD, Helen EverettiD, Janet Mary DalyiD, Nicole C. RobbiD
Abstract
The Orthomyxoviridae family includes influenza D virus (IDV), an emerging pathogen primarily affecting cattle and swine, with evidence of cross-species transmission and potential zoonotic risk. Although active human infections have yet to been confirmed, high seroprevalence in cattle-exposed populations highlights the need for continued surveillance. Here, a rapid, field-deployable RT-LAMP assay for IDV detection was developed and validated, with 99.2% specificity and sensitivity ranging from 95.6% (Cq < 30) to 81.8% (Cq < 40). This method offers a cost-effective, accessible alternative to RT-qPCR, enabling improved monitoring of IDV, and reinforcing preparedness for emerging influenza threats.
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