R. Dijkman

Globoside and the mucosal pH mediate parvovirus B19 entry through the epithelial barrier

This study shows that parvovirus B19 uses globoside and acidic pH to enter epithelial cells via transcytosis, revealing a novel mechanism for respiratory transmission.

The spike gene is a major determinant for the SARS-CoV-2 Omicron-BA.1 phenotype

This study shows the spike gene determines replication, pathogenicity, and immune evasion of SARS-CoV-2 Omicron-BA.1 compared to Delta in cell cultures and animal models.

Establishment of well-differentiated camelid airway cultures to study Middle East respiratory syndrome coronavirus

This study establishes camelid airway epithelial cell cultures to study MERS-CoV infection and replication, showing divergent tropism and interferon inhibition.

Enhanced fitness of SARS-CoV-2 variant of concern B.1.1.7, but not B.1.351, in animal models

The paper compares the fitness of SARS-CoV-2 variants B.1.1.7 and B.1.351 in animal models, finding B.1.1.7 has a fitness advantage in ferrets and mice but not in hamsters.

Effective interferon (IFN)-lambda treatment regimen to control lethal MERS-CoV infection in mice

This study identifies an effective two-dose IFN-lambda regimen that protects mice from lethal MERS-CoV infection by reducing viral load and inflammation.

A genome-wide CRISPR screen identifies interactors of the autophagy pathway as conserved coronavirus targets

This study identifies autophagy-related host factors as conserved targets for coronavirus replication using CRISPR screens and shows that immunophilin inhibitors can block viral replication.

Establishment of a reverse genetic system from a bovine derived Influenza D virus isolate.

This paper establishes a reverse genetic system for a bovine Influenza D virus isolate to study its host tropism.

Betulonic acid derivatives inhibiting coronavirus replication in cell culture via the nsp15 endoribonuclease

This study identifies betulonic acid derivatives as inhibitors of coronavirus replication by targeting the nsp15 endoribonuclease, with EC50 of 0.6 M against 229E.

Trimeric SARS-CoV-2 Spike proteins produced from CHO-cells in bioreactors are high-quality antigens

This paper describes a scalable method to produce high-quality trimeric SARS-CoV-2 Spike proteins for vaccines and diagnostics.

Susceptibility of well-differentiated airway epithelial cell cultures from domestic and wildlife animals to SARS-CoV-2

This study assesses the susceptibility of airway epithelial cell cultures from various animals to SARS-CoV-2, finding efficient replication only in monkey and cat models.

SARS-CoV-2 spike D614G variant confers enhanced replication and transmissibility

This study shows the SARS-CoV-2 D614G variant has enhanced replication and transmissibility in animal models, explaining its global dominance.

LY6E impairs coronavirus fusion and confers immune control of viral disease

This study identifies LY6E as an interferon-induced gene that restricts coronavirus entry and pathogenesis by inhibiting spike protein-mediated fusion and protecting immune cells.

Identification of five antiviral compounds from the Pandemic Response Box targeting SARS-CoV-2

This study screens 400 compounds from the Pandemic Response Box and identifies five with antiviral activity against SARS-CoV-2, including some more effective than Remdesivir.

Disparate temperature-dependent virus - host dynamics for SARS-CoV-2 and SARS-CoV in the human respiratory epithelium

This study compares temperature-dependent replication and immune response dynamics of SARS-CoV-2 and SARS-CoV in human airway epithelial cells, highlighting differences in their antiviral responses and transmission efficiency.

Comprehensive single cell analysis of pandemic influenza A virus infection in the human airways uncovers cell-type specific host transcriptional signatures relevant for disease progression and pathogenesis.

This study uses scRNA-seq to analyze IAV infection in human airway epithelial cells, revealing cell-type specific host responses and viral tropism shifts.

Efficient inactivation of SARS-CoV-2 by WHO-recommended hand rub formulations and alcohols

The paper tests the virucidal activity of WHO-recommended hand rubs and alcohols against SARS-CoV-2, showing efficient inactivation for all tested products.

Rapid reconstruction of SARS-CoV-2 using a synthetic genomics platform

This paper describes a yeast-based synthetic genomics platform for rapid reconstruction of RNA viruses, including SARS-CoV-2, using synthetic DNA fragments and TAR cloning.

Establishment of primary transgenic human airway epithelial cell cultures to study respiratory virus-host interactions

This study establishes a method to genetically modify primary human airway epithelial cells to study virus-host interactions without affecting innate immune responses or viral propagation.

Determining the replication kinetics and cellular tropism of the ruminant-associated Influenza D virus on primary human airway epithelial cells

This study investigates the replication and cell tropism of Influenza D virus in human airway epithelial cells, finding efficient replication and ciliated cell tropism similar to Influenza C virus.

Determination of host cell proteins constituting the molecular microenvironment of coronavirus replicase complexes by proximity-labeling

The paper identifies host proteins near coronavirus replicase complexes using proximity-labeling and shows their roles in viral replication and host processes.