Microbiology Galaxy Lab: The first community-driven gateway for reproducible and FAIR analysis of microbial data
Engy Mohamed Taha NasriD, Nikolaos PechlivanisiD, Nikolaos StrepisiD, Pierre AmatoiD, Matthias BerntiD, Anshu BhardwajiD, Daniel J BlankenbergiD, Daniela BritesiD, Fabio CumboiD, Katherine DoiD, Emanuele FerrariiD, Timothy J. GriffiniD, Björn Andreas GrüningiD, Saskia HiltemanniD, Cameron J. HydeiD, Pratik Dilip JagtapiD, Ashwani Kumar, Muneer Ahmad MallaiD, Subina MehtaiD, Kimberly L. Kanapeckas MétrisiD, Saim MominiD, Tiffanie Nelson, Asime ObaiD, Christina PavloudiiD, Raphaëlle PéguilhaniD, , Bérénice BatutiD
Abstract
The explosion of microbial omics data has outpaced the ability of many researchers to analyze it, with complex tools and limited computational resources creating barriers to discovery. To address this gap, we present the Microbiology Galaxy Lab: a free, globally accessible, community-supported platform that combines state-of-the-art analytical power with user-friendly accessibility. Supported by the Galaxy and global microbiology communities, this platform integrates over 315 tool suites and 115 curated workflows, enabling comprehensive metabarcoding, (meta)genomic, (meta)transcriptomic, and (meta)proteomic data analysis within a FAIR-aligned environment. It also supports research in the health and infectious disease sectors, as well as in environmental microbiology. The platforms utility is exemplified through various use cases, including antimicrobial resistance tracking, biomarker prediction, microbiome classification, and functional annotation of key microbes. Built on reproducibility and community engagement, it supports creation, sharing, and updating of best-practice workflows. Over 35 tutorials and learning paths empower scientists, fostering an ecosystem that keeps resources at the forefront of microbial science. The Microbiology Galaxy Lab enables collective analysis, democratising research, thereby accelerating discovery across the global microbiology community (microbiology.usegalaxy.org, microbiology.usegalaxy.eu, microbiology.usegalaxy.org.au, microbiology.usegalaxy.fr).
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