Interplay between MYZUS PERSICAE-INDUCED LIPASE 1 and OPDA signaling in controlling green peach aphid infestation on Arabidopsis thaliana

MYZUS PERSICAE-INDUCED LIPASE1 (MPL1) encodes a lipase in Arabidopsis thaliana that is required for controlling infestation by the green peach aphid (GPA; Myzus persicae), an important phloem sap-consuming insect pest. Previously, we demonstrated that MPL1 expression was upregulated in response to GPA infestation, and GPA fecundity was higher on the mpl1 mutant, compared to the wild-type (WT), and lower on 35S:MPL1 plants that constitutively expressed MPL1 from the 35S promoter. Here, we show that the MPL1 promoter is active in the phloem and expression of the MPL1 coding sequence from the phloem-specific SUC2 promoter is sufficient to restore resistance to the GPA in the mpl1 mutant. The GPA infestation-associated upregulation of MPL1 requires CYCLOPHILIN 20-3 (CYP20-3), which encodes a 12-oxo- phytodienoic acid (OPDA)-binding protein that is involved in OPDA signaling and is required for controlling GPA infestation. OPDA promotes MPL1 expression to limit GPA fecundity, a process that requires CYP20-3 function. These results along with our observation that constitutive expression of MPL1 from the 35S promoter restores resistance to the GPA in the cyp20-3 mutant, and MPL1 feedbacks to limit OPDA levels in GPA-infested plants, suggest that an interplay between MPL1, OPDA, and CYP20-3 contributes to resistance to the GPA. HighlightInteraction between MYZUS PERSICAE-INDUCED LIPASE 1 function in the phloem, and 12-oxo-phytodienoic acid (OPDA) and CYCLOPHILIN 20-3, which encodes an OPDA-binding protein that is involved in OPDA signaling, is involved in controlling green peach aphid infestation on Arabidopsis thaliana.