Identification of β-synuclein on secretory granules in chromaffin cells and the effects of α- and β-synuclein on BDNF discharge following fusion

Synuclein is strongly implicated in the pathogenesis of Parkinsons disease as well as in other neurodegenerative diseases. However, its normal function in cells is not understood. The N-termini of -, {beta}-, and {gamma}-synuclein are comprised of seven 11-amino acid repeats that are predicted to form amphipathic helices. -Synuclein binds to negatively charged lipids, especially small vesicles and tubulates and vesiculates lipids. The membrane-binding and membrane-curving abilities raise the possibility that synuclein could alter cellular processes that involve highly curved structures. In the present study we examined the localization of endogenous synuclein in bovine chromaffin cells by immunocytochemistry and its possible function to control protein discharge upon fusion of the granule with the plasma membrane by regulating the fusion pore. We found with quantitative immunocytochemistry that endogenous {beta}-synuclein associates with secretory granules. Endogenous -synuclein only rarely is found on secretory granules. Overexpression of -synuclein but not {beta}-synuclein quickened the median duration of the post-fusion discharge of BDNF-pHluorin by 30%, consistent with -synuclein speeding fusion pore expansion.