Genome biologyBioinformaticsPeer-reviewed journal article
AlleleAnalyzer: a tool for personalized and allele-specific sgRNA design
Kathleen C. KeoughiD, Svetlana LyalinaiD, Michael P. Olvera, Sean WhaleniD, Bruce R. ConkliniD, Katherine S. PollardiD
Abstract
The CRISPR/Cas system is a highly specific genome editing tool capable of distinguishing alleles differing by even a single base pair. However, current tools only design sgRNAs for a reference genome, not taking into account individual variants which may generate, remove, or modify CRISPR/Cas sgRNA sites. This may cause mismatches between designed sgRNAs and the individual genome they are intended to target, leading to decreased experimental performance. Here we describe AlleleAnalyzer, a tool for designing personalized and allele-specific sgRNAs for genome editing. We leverage >2,500 human genomes to identify optimized pairs of sgRNAs that can be used for human therapeutic editing in large populations in the future.
- organism: human
- assay: CRISPR screen
- research focus: software / tool
- research focus: variant calling
- research focus: gene regulatory network inference
- approach: statistical model
- approach: experimental assay
- Citations
- 26
- FWCI
- 1.32
- Field percentile
- top 20%
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