The anticancer activity and immune modulating properties of combinatorial ensemble complementary (antisense) microRNA (fRNA) in combination with the immunomodulator - glycoproteid lectin from B. subtilis B-7025

Abstract

BackgroundMany adenocarcinomas have the ability to capture from an extracellular matrix the oligonucleotides and nanoparticles by pinocytosis, when the non-cancerous cells are not capable to capture the oligonucleotides and small liposomes. This provides selective accumulation of proposed protected oligonucleotides (fRNA) in cancer cells and also provides the absence toxicity in the fRNA.\n\nMaterials and methodsFor the immunotherapy we used immunotropic 70 kDa lectin B. subtilis B-7025. In vivo experiments were carried out in C57BL line mice in Lewis lung carcinoma. The cytotoxic activity, lymphocytes, macrophages was determined in vitro using the MTT assay.\n\nResults and discussionAnimal survival rate in groups receiving either the fRNA or Vaccine was 70 and 40%, respectively. Combined use fRNA and Vaccine has the advantage compared with the use these drugs in monotherapy, as the anticancer efficacy of the scheme is much higher, which is manifested in the primary tumor node and metastasis inhibition.