bioRxivImmunology
Early induction of SARS-CoV-2 specific T cells associates with rapid viral clearance and mild disease in COVID-19 patients
A. T. Tan, M. Linster, C. Tan, N. Le Bert, W. Chia, K. Kunasegaran, Y. Zhuang, C. Y. Tham, A. Chia, G. J. Smith, B. E. Young, S. Kalimuddin, J. G. Low, D. Lye, L.-F. Wang, A. Bertoletti
Abstract
Virus-specific humoral and cellular immunity act synergistically to protect the host from viral infection. We interrogated the dynamic changes of virological and immunological parameters in 12 patients with symptomatic acute SARS-CoV-2 infection from disease onset to convalescence or death. We quantified SARS-CoV-2 viral RNA in the respiratory tract in parallel with antibodies and circulating T cells specific for various structural (NP, M, ORF3a and spike) and non-structural proteins (ORF7/8, NSP7 and NSP13). We observed that while rapid induction and quantity of humoral responses were associated with increased disease severity, an early induction of SARS-CoV-2 specific T cells was present in patients with mild disease and accelerated viral clearance. These findings provide further support for a protective role of SARS-CoV-2 specific T cells over antibodies during SARS-CoV-2 infection with important implications in vaccine design and immune-monitoring.
- organism: human
- assay: bulk RNA-seq
- assay: proteomics
- research focus: host-pathogen & immune response
- research focus: cell type annotation
- research focus: differential expression
- approach: experimental assay
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- Early antiviral CD4 and CD8 T cell responses and antibodies are associated with upper respiratory tract clearance of SARS-CoV-2
S. I. Ramirez, P. G. Lopez, … ACTIV-2/A5401 Study Team·bioRxiv·Oct 25, 2023
- Highly functional virus-specific cellular immune response in asymptomatic SARS-CoV-2 infection
N. Le Bert, H. E. Clapham, … C. C. Tam·bioRxiv·Nov 25, 2020
- Deciphering the quality of SARS-CoV-2 specific T-cell response associated with disease severity, immune memory and heterologous response
A. Perez-Gomez, M. C. Gasca-Capote, … E. Ruiz-Mateos·bioRxiv·Dec 28, 2021
- Divergent SARS-CoV-2-specific T and B cell responses in severe but not mild COVID-19
A. E. Oja, A. Saris, … P. Hombrink·bioRxiv·Jun 18, 2020
- Ex vivo detection of SARS-CoV-2-specific CD8+ T cells: rapid induction, prolonged contraction, and formation of functional memory
I. Schulien, J. Kemming, … C. Neumann-Haefelin·bioRxiv·Aug 13, 2020
- Rapid induction of antigen-specific CD4+ T cells guides coordinated humoral and cellular immune responses to SARS-CoV-2 mRNA vaccination
M. M. Painter, D. Mathew, … E. J. Wherry·bioRxiv·Apr 21, 2021