Nature CommunicationsSynthetic BiologyPeer-reviewed journal article
Engineering species-like barriers to sexual reproduction
Maciej MaselkoiD, Stephen C. HeinschiD, Jeremy M. ChacóniD, William R. HarcombeiD, Michael J. SmanskiiD
Abstract
We introduce a novel approach to engineer a genetic barrier to sexual reproduction between otherwise compatible populations. Programmable transcription factors drive lethal gene expression in hybrid offspring following undesired mating events. As a proof of concept, we target the ACT1 promoter of the model organism Saccharomyces cerevisiae using a dCas9-based transcriptional activator. Lethal over-expression of actin results from mating this engineered strain with a strain containing the wild-type ACT1 promoter.
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