MAPseq2: a sensitive and cost-effective barcoded connectomics method
Hyopil KimiD, Huihui QiiD, Craig Washington, Xiao Yang LiangiD, Justus M. KebschulliD
Abstract
The barcoded connectomics tool MAPseq enables highly multiplexed projection mapping of individual neurons by translating neuroanatomy into a format solvable by DNA sequencing. Here we present MAPseq2, a user-friendly protocol with up to [~]10-fold increased sensitivity compared to the current state of the art and [~]10-fold decreased cost. As MAPseq workflows are used across a range of barcoded connectomics methods, including BARseq, BRICseq, and ConnectID, all improvements in MAPseq2 directly transfer to these technologies.
02 /Specification & Classification
Click any tag to browse matching papers- Citations
- 2
- Approach
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- Using high-throughput barcode sequencing to efficiently map connectomes
Ian D. Peikon, Justus M. Kebschull, … Anthony M. Zador·Nucleic Acids ResearchPeer-reviewed·Apr 13, 2017
- High-throughput mapping of single neuron projections by sequencing of barcoded RNA
Justus M Kebschull, Pedro Garcia da Silva, … Anthony M Zador·NeuronPeer-reviewed·Sep 1, 2016
- Expansion Sequencing of RNA Barcoded Neurons in the Mammalian Brain: Progress and Implications for Molecularly Annotated Connectomics
Daniel Goodwin, Alex Vaughan, … Richie E. Kohman·bioRxiv·Jul 31, 2022
- High-throughput mapping of long-range neuronal projection using in situ sequencing
Xiaoyin Chen, Yu-Chi Sun, … Anthony M. Zador·CellPeer-reviewed·Oct 1, 2019
- A computational framework for converting high-throughput DNA sequencing data into neural circuit connectivity.
Hassana K. Oyibo, Cang Cao, … Anthony M. Zador·bioRxiv·Jan 7, 2018
- Conneconomics: The Economics of Dense, Large-Scale, High-Resolution Neural Connectomics
Adam H. Marblestone, Evan R. Daugharthy, … George M. Church·bioRxiv·Apr 21, 2014