Hi-C yields chromosome-length scaffolds for a legume genome, Trifolium subterraneum

Olga Dudchenko; Melanie Pham; Christopher G. Lui; Sanjit Singh Batra; Marie Hoeger; Sarah K. Nyquist; Neva C. Durand; Muhammad S. Shamim; Ido Machol; W. Erskine; Erez Lieberman Aiden; Parwinder Kaur

doi:10.1101/473553

GenomicsbioRxivNov 20, 2018

Preprint

Hi-C yields chromosome-length scaffolds for a legume genome, Trifolium subterraneum

Olga Dudchenko iD, Melanie Pham iD, Christopher G. Lui iD, Sanjit Singh Batra iD, Marie Hoeger iD, Sarah K. Nyquist iD, Neva C. Durand iD, Muhammad S. Shamim iD, Ido Machol iD, W. Erskine iD, Erez Lieberman Aiden iD, Parwinder Kaur iD

Abstract

We present a chromosome-length assembly of the genome of subterranean clover, Trifolium subterraneum, a key Australian pasture legume. Specifically, in situ Hi-C data (48X) was used to correct misjoins and anchor, order, and orient scaffolds in a previously published genome assembly (TSUd_r1.1; scaffold N50: 287kb). This resulted in an improved genome assembly (TrSub3; scaffold N50: 56Mb) containing eight chromosome-length scaffolds that span 95% of the sequenced bases in the input assembly.

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