NMDAR-activated PP1 dephosphorylates GluN2B to modulate NMDAR synaptic content.

Abstract

In mature neurons, postsynaptic NMDARs are segregated into two populations, synaptic and extrasynaptic, which differ in localization, function, and associated intracellular cascades. These two pools are connected via lateral diffusion, and receptor exchange between them modulates synaptic NMDAR content. Here, we identify the phosphorylation of the PDZ-ligand of the GluN2B subunit of NMDARs (at S1480) as a critical determinant in dynamically controlling NMDAR synaptic content. We find that phosphorylation of GluN2B at S1480 maintains NMDARs at extrasynaptic membranes as part of a protein complex containing Protein Phosphatase 1 (PP1). Global activation of NMDARs leads to the activation of PP1, which mediates dephosphorylation of GluN2B at S1480 to promote an increase in synaptic NMDAR content. Thus, PP1-mediated dephosphorylation of the GluN2B PDZ-ligand modulates the synaptic expression of NMDARs in mature neurons in an activity-dependent manner, a process with profound consequences for synaptic and structural plasticity, metaplasticity, and synaptic neurotransmission.\n\nHIGHLIGHTSO_LIPhosphorylation of the PDZ-ligand of the GluN2B subunit of NMDARs (GluN2B-pS1480) maintains NMDARs at extrasynaptic sites.\nC_LIO_LIExtrasynaptic NMDARs form a stable protein complex containing PP1.\nC_LIO_LIGlobal NMDAR activation increases NMDAR synaptic content by promoting PP1-mediated dephosphorylation of GluN2B-pS1480.\nC_LIO_LIGluN2B-pS1480 dephosphorylation is mediated by a PP1 subpopulation not involved in LTD and is enhanced by age.\nC_LI