Upregulation of Inhibitor of DNA Binding 1 and 3 is Important for Efficient Thermogenic Response in Human Adipocytes

Brown and beige adipocytes can be activated by {beta}-adrenergic agonist via cAMP-dependent signaling. Performing RNA-sequencing analysis in human cervical area-derived adipocytes, we found that dibutyryl-cAMP, which can mimic in vivo stimulation of browning and thermogenesis, enhanced the expression of browning and batokine genes and upregulated several signaling pathway genes linked to thermogenesis. We observed that the expression of Inhibitor of DNA binding and cell differentiation (ID) 1 and particularly ID3 was strongly induced by the adrenergic stimulation. The degradation of ID1 and ID3 elicited by the ID antagonist AGX51 during thermogenic activation prevented the induction of proton leak respiration that reflects thermogenesis and abrogated cAMP analogue-stimulated upregulation of thermogenic genes and mitochondrial complex I, II, and IV subunits. The presented data suggests that ID proteins contribute to efficient thermogenic response of adipocytes during adrenergic stimulation. Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=117 SRC="FIGDIR/small/601234v1_ufig1.gif" ALT="Figure 1"> View larger version (16K): org.highwire.dtl.DTLVardef@bf8235org.highwire.dtl.DTLVardef@fd89dcorg.highwire.dtl.DTLVardef@165eeedorg.highwire.dtl.DTLVardef@12921ea_HPS_FORMAT_FIGEXP M_FIG C_FIG