Stem CellsGeneticsPeer-reviewed journal article
Cell cycle dynamics of human pluripotent stem cells primed for differentiation
Anna ShcherbinaiD, Jingling Li, Cyndhavi NarayananiD, William James GreenleafiD, Anshul Bharat KundajeiD, Sundari ChettyiD
Abstract
Understanding the molecular properties of the cell cycle of human pluripotent stem cells (hPSCs) is critical for effectively promoting differentiation. Here, we use the Fluorescence Ubiquitin Cell Cycle Indicator (FUCCI) system adapted into hPSCs and perform RNA-sequencing on cell cycle sorted hPSCs primed and unprimed for differentiation. Gene expression patterns of signaling factors and developmental regulators change in a cell cycle-specific manner in cells primed for differentiation without altering genes associated with pluripotency. Furthermore, we identify an important role for PI3K signaling in regulating the early transitory states of hPSCs towards differentiation.
- organism: human
- assay: bulk RNA-seq
- research focus: differential expression
- research focus: gene regulatory network inference
- approach: statistical model
- Citations
- 9
- FWCI
- 0.58
- Field percentile
- top 40%
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