Nature CommunicationsSystems BiologyPeer-reviewed journal article
Reliable identification of protein-protein interactions by crosslinking mass spectrometry
Swantje LenziD, Ludwig Roman SinniD, Francis J. O’ReillyiD, Lutz FischeriD, Fritz WegneriD, Juri RappsilberiD
Abstract
Crosslinking mass spectrometry is widening its scope from structural analyzes of purified multi-protein complexes towards systems-wide analyzes of protein-protein interactions. Assessing the error in these large datasets is currently a challenge. Using a controlled large-scale analysis of Escherichia coli cell lysate, we demonstrate a reliable false-discovery rate estimation procedure for protein-protein interactions identified by crosslinking mass spectrometry.
- organism: E. coli
- assay: proteomics
- research focus: variant effect prediction
- approach: statistical model
- Citations
- 157
- FWCI
- 9.21
- Field percentile
- top 1%
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