Ludwig Roman Sinn

The paper shows that alpha-synuclein phase separation can form Lewy body-like structures without pre-existing seeds, suggesting a new mechanism for neurodegeneration.

The paper creates a proteoform-resolved atlas of the human plasma proteome, revealing extensive proteoform diversity and highlighting discrepancies between proteomic platforms.

SILAC-Site directly measures phosphosite stoichiometries without enrichment, showing low stoichiometry for most phosphopeptides and correlating with direct detection.

Pho-Tip is a one-pot dephosphorylation method that improves the detection of low-abundant phosphorylated peptides in DIA phosphoproteomics data.

The paper evaluates ZT Scan DIA, a proteomics method combining scanning DIA with ion trapping, showing improved precursor and protein identification, quantification, and bias reduction in multi-species mixtures.

The paper observes decreased molecular crowding during yeast to filamentous growth transition in C. albicans, linked to reduced ribosome concentration and increased cytoplasmic volume.

The paper benchmarks proteomics accuracy using a large-scale dataset, identifying systematic errors and providing a resource for method optimization and software evaluation.

This study shows that fluconazole tolerance in fungi is linked to decreased cytoplasmic fluidity and increased heterogeneity, mediated by changes in ribosome dynamics and P-body formation.

The paper shows phosphorylation modulates deadenylation rates via multivalent interactions in RNA adaptors, enabling graded regulation of mRNA decay.

This paper improves crosslinking mass spectrometry by using diazirine-based photo-crosslinking to better study dynamic protein regions and their conformational changes.

QuantUMS, a machine-learning module in DIA-NN, weights MS1 and fragment signals by quality-derived variance estimates, improving DIA precision, removing ratio compression in high-accuracy mode, and scoring each quantity's reliability.

The paper evaluates MS-cleavable crosslinkers, finding that improved peptide backbone fragmentation, not mass resolution, is the main advantage of DSSO.

The paper demonstrates a reliable false-discovery rate estimation method for protein-protein interactions identified by crosslinking mass spectrometry using Escherichia coli cell lysate.

The paper uses a neural network to predict retention times in crosslinking mass spectrometry, improving protein-protein interaction identification.

The paper determines the in-cell architecture of a transcribing and translating expressome in Mycoplasma pneumoniae using integrative structural methods.

This study shows that Escherichia coli can adapt to use fluorinated indoles as essential precursors for protein synthesis through few genetic mutations and significant regulatory and cellular changes.

This study investigates the structural architecture of two SMC-kleisin complexes, revealing a folded conformation mediated by a dynamic coiled-coil discontinuity.

The paper identifies non-covalently-associated peptides in mass spectrometry that can mislead crosslink analysis and proposes methods to distinguish them from true crosslinks.