Claudio Iacobucci

Revisiting p53:Sirt1 interaction in the light of controlling p53 acetylation levels

This study presents the first structural model of the full-length p53:Sirt1 complex, showing Sirt1 selectively deacetylates p53 at K582 and binds acetylated p53 with a 1:1 stoichiometry.

Structural studies of the C-terminal cysteine-rich domain of Aedes albopictus vitellogenin reveal an evolutionarily divergent, monomeric C-terminal cysteine knot (CTCK) architecture

This study determines the crystal structure of the C-terminal cysteine knot domain of Aedes albopictus vitellogenin, revealing a monomeric, evolutionarily divergent architecture that challenges prior assumptions about its oligomerization role.

Enantiopurity-Dependent Peptide Coacervates and Asymmetric Organocatalysis

This study shows that enantiopure peptide coacervates form stable liquid droplets and exhibit enantioselective catalytic activity, while racemic mixtures form solid precipitates.

Protein Secondary Structure Patterns In Short-Range Cross-Link Atlas

The paper analyzes short-range cross-links to reveal their correlation with protein secondary structure and introduces X-SPAN for integrating XL-MS data with AlphaFold predictions.

A community audit of error control in crosslinking mass spectrometry

The paper audits error control in twelve crosslinking mass spectrometry pipelines using ground-truth designs, finding some pipelines exceed false discovery rates by up to 29-fold.

Molecular Structure and Enzymatic Mechanism of the Human Collagen Hydroxylysine Galactosyltransferase GLT25D1/COLGALT1

This study characterizes the molecular structure and enzymatic mechanism of GLT25D1/COLGALT, identifying its catalytic site and dimeric assembly as critical for collagen lysine modification.

Twisting Urea- to Imide-Based Mass Spectrometry-Cleavable Cross-Linkers Enables Affinity Tagging

This paper introduces DSSI, a new class of MS-cleavable cross-linkers with affinity tags, and validates its use in structural biology.

A Workflow for Improved Analysis of Cross-linking Mass Spectrometry Data Integrating Parallel Accumulation-Serial Fragmentation with MeroX and Skyline

This paper introduces a workflow for improved XL-MS data analysis using PASEF, integrating MeroX and Skyline for better identification of cross-linked products.

A Simple Cross-Linking/Mass Spectrometry Workflow to Study System-Wide Protein Interactions

The paper introduces a streamlined XLMS workflow and updated software tool for proteome-wide protein interaction analysis, demonstrating results in Drosophila embryos.

Cross-linking/Mass Spectrometry: A Community-Wide, Comparative Study Towards Establishing Best Practice Guidelines

This paper summarizes current XL-MS practices, compares strategies, and proposes common protocols to establish best practice guidelines for accurate and reproducible results.