W. Roseboom

Non-mechanical disruption of Bacillus subtilis spores allows sensitive and deep characterization of the minimal proteome for resuming a cellular lifestyle.

This paper introduces a new sample preparation method, SPEED, which improves proteomic analysis of Bacillus subtilis spores by identifying more proteins and enabling differentiation between spore and vegetative cell proteins.

Multi-omics analysis of Bacillus. subtilis spores formed at different environmental temperatures reveal differences at the morphological and molecular level.

This study analyzes B. subtilis spores formed at different temperatures to identify molecular and morphological differences.

Integrative metabolomics and proteomics allow the global intracellular characterization of Bacillus subtilis cells and spores.

This study uses metabolomics and proteomics to characterize B. subtilis vegetative cells and spores, showing differences between them and demonstrating the effectiveness of the 60% EtOH extraction protocol.

Whitefly effector G4 interacts with tomato proteins of which MIPDB141 affects whitefly performance

The paper identifies effector G4 from whitefly Bemisia tabaci and shows it suppresses tomato immunity by interacting with MIPDB141, reducing whitefly fecundity.

Cross-link scrambling in peptide pairs

The paper identifies cross-link types in peptides using SCXC retention time prediction and shows that MS/MS analysis reveals ester linkages in some amide-predicted pairs due to gas-phase rearrangement.

High resolution analysis of proteome dynamics during Bacillus subtilis sporulation

This study uses high-resolution time-lapse proteomics to analyze proteome dynamics during Bacillus subtilis sporulation, identifying co-expressed modules and potential new sporulation-related proteins.

A composite filter for low FDR of protein-protein interactions detected by in vivo cross-linking

The paper introduces a composite filter to reduce the false discovery rate of protein-protein interactions detected by in vivo cross-linking using BAMG and pLink 2.

The Ice Nucleating Protein InaZ is Activated by Low Temperature

This study investigates how the ice-nucleating protein InaZ from Pseudomonas syringae folds and interacts with water at low temperatures, revealing structural changes that enhance ice nucleation.

Towards the minimal proteome of life: Proteome profiles of Bacillus subtilis vegetative cells and spores

This study quantifies protein levels in Bacillus subtilis vegetative cells and spores using 15N metabolic labeling, revealing 60% shared proteins essential for spore survival and germination.

Multiple Duration Priors Within and Across the Senses

The paper shows humans can acquire and maintain multiple duration priors based on sensory signal type, both within and across senses.

In culture cross-linking of bacterial cells reveals proteome scale dynamic protein-protein interactions at the peptide level

The paper develops a method to identify dynamic protein-protein interactions at the peptide level in bacteria using cross-linking in culture, revealing new interactions in transcription and translation.