Methodology for isolation of serum, cerebrospinal fluid, and hippocampal neuron proteins from rat and their analysis using mass spectrometry-based shotgun proteomics

Abstract

Emerging interests in the field of research related to diseases such as Alzheimers disease, Parkinsons disease, cognition, and other mental health-related disorders have prompted a need for a common method for the isolation of serum, CSF, and hippocampus. The hippocampus is responsible for learning and memory. It can be affected by various neurological and psychiatric disorders. However, the process of collecting samples such as CSF and hippocampal neurons is challenging, especially for small animals like rats. We have presented here a method for the isolation of serum, CSF, and hippocampal neurons that can be used for its downstream applications such as proteomics. We have used high-speed centrifugation instruments and density gradient centrifugation methods, which are easy to follow. Additionally, we have tested the proteins identified through mass spectrometry. Our method enables the study of proteins in serum, CSF, and neural cells for researching protein cross-talks and neurological disorder mechanisms.