Viviana Gradinaru

This study demonstrates that AAV-DJ efficiently and safely transduces human ovarian cells, enabling targeted gene modulation with significant knockdown of AMHR2 and TGF{beta}1.

This study shows that Hrs, an ESCRT-0 protein, modulates AMPA receptor currents and Ca2+-dependent signaling by sensing and responding to neuronal activity at synapses.

The paper identifies AAV vectors that efficiently deliver transgenes to the nervous systems of spiny mice, enabling functional studies of regeneration.

The paper uses Perturb-seq in mice to identify genes that promote collateral artery formation by comparing gene expression between guinea pigs and mice.

The paper establishes a method for in utero genome editing in fetal mouse cortex at midgestation, demonstrating efficient editing with reduced off-target effects.

This paper introduces a method to link AAV capsids for coordinated delivery of genetic cargo, improving efficiency and uniformity in gene delivery.

The paper solves cryo-EM structures of AAV capsids and their complexes with AAVR to design variants with reduced liver targeting and altered brain cell tropism.

The paper introduces DIMMERs, miRNA-based circuits that enable precise, tunable control of transgene expression across cell types, improving gene therapy and research applications.

This study models Parkinson's disease in spiny mice, showing they exhibit key disease features like behavioral impairments, neuronal degeneration, and proteomic changes similar to PD.

This study engineers AAVs targeting human carbonic anhydrase IV to enhance brain gene delivery, finding that optimized variants achieve 100-fold greater transduction than AAV9.

This study shows that the gut microbiome increases mitochondrial respiration and oxidative stress in the brain of a Parkinson's disease mouse model, worsening motor symptoms.

This paper introduces three HCR variants that improve sensitivity for detecting low-abundance mRNA targets in challenging tissue environments.

The paper identifies transcriptional crosstalk between enhancers and promoters delivered by AAVs, showing concatemer formation enables targeted delivery of large genetic cargo for cell-type-specific gene editing.

The paper introduces a programmable self-assembling protein system to model intracellular protein aggregation in neurodegenerative diseases and reveals disease-specific aggregate-organelle interactions.

The paper introduces ADePT, a method for mapping neural circuit functional connectivity using optogenetic stimulation and two-photon imaging in mice.

The paper applies molecularly-guided single-cell spatial proteomics to study proteomic differences in healthy and diseased mammalian brains, revealing insights into Parkinson's disease.

A synthetic miRNA-based incoherent feed-forward loop circuit embedded in a gene delivery vector overcomes the challenge of overexpression toxicity in a mouse model of Rett syndrome gene therapy.

This study removes a genomic duplication in a mouse model using DN-CRISPRs, improving synaptic transmission and behavior.

The paper shows that timed activation of VIP neurons in the SCN and late afternoon light exposure can rescue dysregulated estrus cycles in mice.

This study shows that dorsal raphe dopamine neurons encode motivational salience, responding to reward and punishment cues and modulated by internal and external states.

This study identifies the mechanism of first lineage segregation in human embryos through cell polarisation, showing it occurs at the 8-cell stage via F-actin and Par complex polarisation, with PLC activation and GATA3 regulation.

The paper develops enhancer viruses and a transgenic mouse line to enable combinatorial labeling of specific neuronal subclasses in the mouse cortex and human slices.

The paper identifies enhancer elements that drive subclass-specific gene expression in neocortex across mouse, primate, and human species using comparative epigenetics and AAV-based functional assays.

The paper introduces a light-inducible recombinase system for targeted genomic modifications in neurons, enabling precise labeling and functional analysis of neural circuits in live mouse brains.

The paper identifies peripheral neural circuits regulating heart rate in mice using optogenetic and viral vector strategies, revealing distinct roles of cholinergic and noradrenergic neurons.

This paper describes a protocol for producing and administering AAV-PHP vectors to label and manipulate cells in the mouse nervous system and organs, with guidelines for experimental design.