ANYI: The ANnotated Yeast Interactome

Although several existing protein-protein interaction (PPI) databases provide yeast PPI data, none unify large-scale network topology information with detailed biophysical, proteostasis, and regulatory annotations in a single protein-centric framework. To address this gap, we developed the ANnotated Yeast Interactome (ANYI), an open, integrated resource that combines experimental yeast PPIs with sixteen feature annotation types, including protein abundance, half-life, disorder content, post-translational modifications, conformational stability, chaperone interactions, sequence, and structure. ANYI integrates 3,927 proteins with 155 annotation features, forming a unified matrix that enables systematic cross-layer analyses. Available via GitHub and Docker Hub with an interactive network browser for broad accessibility, ANYI provides both experienced and beginner computational scientists with tools to investigate the yeast interactome. For example, users can directly test whether highly connected hub proteins exhibit distinct stability, disorder, or proteostasis signatures relative to peripheral nodes. AVAILABILITY AND IMPLEMENTATIONThe code used to assemble ANYI is available on GitHub at https://github.com/NCEMS/energetic-origins-of-PPI-connectivity and the database itself and interactive browser tool are available on Docker Hub as dannissleypsu/anyi-browser:v1.0.2.