bioRxivBioinformaticsPreprint
The Precise Basecalling of Short-Read Nanopore Sequencing
Ziyuan WangiD, Mei‐Juan TuiD, Chengcheng SongiD, Ziyang LiuiD, Katherine K. WangiD, Shuibing CheniD, Ai-Ming Yu, Hongxu DingiD
Abstract
The nanopore sequencing of short sequences, whose lengths are typically less than 0.3kb therefore comparable with Illumina sequencing techniques, has recently gained wide attention. Here, we design a scheme for training nanopore basecallers that are specialized for short biomolecules. With bioengineered RNA (BioRNA) molecules as examples, we demonstrate the superior accuracy of basecallers trained by our scheme.
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