Generation of mutation hotspots in ageing bacterial colonies
Agnieszka Sekowska, Sofie Wendel, Morten Helge Hauberg Norholm, Antoine Danchin
Abstract
How do ageing bacterial colonies generate adaptive mutants? Over a period of two months, we isolated on ageing colonies outgrowing mutants able to use a new carbon source, and sequenced their genomes. This allowed us to uncover exquisite details on the molecular mechanism behind their adaptation: most mutations were located in just a few hotspots in the genome and over time, mutations increasingly originated from 8-oxo-guanosine, formed exclusively on the transcribed strand.
02 /Specification & Classification
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