Exploiting the sequence diversity of TALE-like repeats to vary the strength of dTALE-promoter interactions

Introduction Introduction Methods Results Discussion SI References TALE (Transcriptional activator like effector) repeat arrays are a popular form of programmable DNA binding domain. Reprogrammed TALE transcription factors are referred to as dTALEs (designer-TALEs), and are used within the field of synthetic biology1 as well as fundamental research2.\n\nNaturally TALEs are pathogenicity factors secreted by Xanthomonas spp. into host plant cells to bind specific promoters and manipulate host transcription3. Sequence specific DNA binding is conferred by the TALE repeat array. Natural TALE repeat arrays are formed of 10-30 repeats4, each of which pairs with one DNA base. Collectively the repeats ...