Javier Estrada

The paper shows that a mutation in a minimal enhancer of Drosophila eve stripe 2 is buffered by flanking sequences in extended enhancer constructs, but not in the endogenous context.

The paper investigates the HbP2 enhancer in Drosophila, showing that cooperative binding of Bcd is insufficient to explain its sharp expression pattern, and identifies other factors like pioneer factors and histone modifiers as key contributors.

The paper shows that Caudal counter-represses Hunchback to enable even-skipped stripe 2 expression in Drosophila embryos, challenging the traditional view of Hunchback as a direct activator.

SiteOut is an online tool that designs DNA sequences without binding sites for specified factors, allowing for precise control over regulatory DNA sequences.

The paper shows that Hunchback regulates two enhancers with opposite activities to produce the same gene expression pattern in Drosophila embryos.