bioRxivBiophysics
A Mean Shift Algorithm for Drift Correction in Localization Microscopy
F. J. Fazekas, T. R. Shaw, S. Kim, R. A. Bogucki, S. L. Veatch
Abstract
Single molecule localization microscopy (SMLM) techniques transcend the diffraction limit of visible light by localizing isolated emitters sampled stochastically. This time-lapse imaging necessitates long acquisition times, over which sample drift can become large relative to the localization precision. Here we present a novel, efficient, and robust method for estimating drift using a simple peak-finding algorithm based on mean shifts that is effective for SMLM in 2 or 3 dimensions.
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