J. Ni

In vivo mRNA delivery to the lung vascular endothelium by dicationic Charge-Altering Releasable Transporters

This study develops dicationic Charge-Altering Releasable Transporters (CARTs) that deliver mRNA specifically to lung endothelial cells via intravenous administration.

Enhanced Recognition of a Herbal Compound Epiberberine by a DNA Quadruplex-Duplex Structure

This paper investigates how epiberberine binds to a DNA quadruplex structure and shows how modifying the DNA structure enhances binding affinity, leading to a new method for extracting the compound from plant extracts.

Optimization of gene knockout approaches and practical solutions to sgRNA selection challenges in hPSCs with inducible Cas9 system

This study optimizes gene knockout in hPSCs using an inducible Cas9 system and identifies effective sgRNAs by comparing experimental results with cleavage efficiency algorithms.

The Chlamydia trachomatis secreted effector CebN targets nucleoporins and Rae1 to antagonize STAT1 nuclear import

This study identifies CebN, a Chlamydia trachomatis effector that targets nucleoporins and Rae1 to disrupt STAT1 nuclear import, aiding bacterial survival.

De novo multi-mechanism antimicrobial peptide design via multimodal deep learning

This paper introduces M3-CAD, a deep learning pipeline for designing multi-mechanism antimicrobial peptides to combat multidrug-resistant organisms, showing effectiveness in a skin wound infection model.

Brg1 regulates fibroblast-myofibroblast transition to promote renal fibrosis

This study shows Brg1 promotes fibroblast-myofibroblast transition in renal fibrosis and identifies Prune2 and PU.1 as downstream targets, with PFI-3 as a potential antifibrotic drug.

Phosphorylated transcription factor PuHB40 is involved in ROS-dependent anthocyanin biosynthesis in pear exposed to high-light stress

This study identifies PuHB40 as a phosphorylation-regulated transcription factor that mediates ROS-dependent anthocyanin biosynthesis in pear under high-light stress.

Breakage fusion bridge cycles drive high oncogene copy number, but not intratumoral genetic heterogeneity or rapid cancer genome change

The paper introduces OM2BFB, an algorithm detecting BFB amplifications using OGMs, showing high precision and recall, and finds BFB events are common in certain cancers but not linked to intratumoral heterogeneity or rapid genome change.

SARS-CoV-2 Omicron infection augments the magnitude and durability of systemic and mucosal immunity in triple-dose CoronaVac recipients

This study examines immune responses in CoronaVac-vaccinated individuals after Omicron infection, showing enhanced antibody and cellular immunity compared to unvaccinated controls.

The canonical single-stranded DNA binding protein SSB is not an essential replication protein but an RNA chaperon in the hyperthermophilic archaeon Saccharolobus islandicus REY15A

This study shows that the canonical SSB in Saccharolobus islandicus REY15A is not essential for DNA replication but functions as an RNA chaperone, with conserved activities in related archaea.

The archaeal KEOPS complex possesses a functional Gon7 homolog and has an essential function independent of cellular t6A modification level

This study identifies the archaeal KEOPS complex subunits and shows its essential function independent of t6A modification.

SP1 affects the migration and invasion of laryngeal cancer cells by regulating methylation of claudin 4 promoter region through the p-JNK pathway

This study shows SP1 regulates CLDN4 promoter methylation via the p-JNK pathway, increasing MMP-2/MMP-9 expression and enhancing laryngeal cancer cell migration and invasion.

A novel RHH family transcription factor aCcr1 and its viral homologs dictate cell cycle progression in archaea

This study identifies aCcr1, an archaeal transcription factor, and its viral homologs as regulators of cell cycle progression, showing they control cell division by repressing cdvA expression.

Identification and calibration of ultrabright localizations to eliminate quantification error in SMLM

This paper identifies and calibrates ultrabright localizations in SMLM to improve quantification accuracy for biomolecular organization.

PeerPub: A device for concurrent operant oral self-administration by multiple rats

PeerPub enables multiple rats to self-administer solutions concurrently, showing increased consumption in group housing compared to isolation.

Seasonality but not photoperiodism affects susceptibility of the two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae) to pesticides

This study shows that seasonality, not photoperiodism, affects the susceptibility of spider mites to pesticides, with toxicity increasing in spring and summer and decreasing in winter.

The African swine fever virus protease pS273R inhibits DNA sensing cGAS-STING pathway by targeting IKKε

This study identifies pS273R as an ASFV protease that inhibits the cGAS-STING pathway by targeting IKKε, revealing a new immune evasion mechanism.

Improving localization precision via restricting biomolecule confined stochastic motion in SMLM

This study investigates stochastic motion of biomolecules in SMLM and shows that restricting this motion improves localization precision.

Targeting EGFR in glioblastoma with a novel brain-penetrant small molecule EGFR-TKI

This paper introduces a novel EGFR-TKI, CM93, which shows improved brain penetration and efficacy against EGFR-mutated glioblastoma in vitro and in vivo.

TailTimer: an open-source device for automating the rodent tail immersion assay

TailTimer automates the rodent tail immersion assay by recording tail withdrawal latency and water temperature, improving reproducibility and detecting genetic differences in thermal pain sensitivity.

Multiple domains in ARHGAP36 regulate PKA degradation and Gli activation

This study identifies how multiple domains in ARHGAP36 regulate PKA degradation and Gli activation through structural and functional analysis.

CM93, a novel covalent small molecule inhibitor targeting lung cancer with mutant EGFR

The paper develops CM93, a novel EGFR inhibitor with improved targeting and brain penetration, showing better efficacy and safety in mouse models of EGFR-mutant NSCLC and brain metastases.

Limited Environmental Serine Confers Sensitivity to PHGDH Inhibition in Brain Metastasis

This study shows that brain metastases rely on PHGDH-mediated serine synthesis due to limited environmental serine, making them sensitive to PHGDH inhibition.

Identification of QTL for leaf angle at canopy-wide levels in maize

The paper identifies dozens of QTL for leaf angle across eight leaves in maize under three environments, revealing genetic architecture differences at various canopy levels.

The socioeconomic burden of severe mental disorder in China, 2014-2017: a prevalence study

This study estimates the economic burden of severe mental disorder in China from 2014-2017 using prevalence-based methods, finding substantial direct and indirect costs.